Samane Abuli; Gholam Reza Dashab; Mohammad Rokouei; Mehdi Vafaei valeh
Volume 17, Issue 1 , April 2015, , Pages 107-117
Abstract
The polymorphism in exon three of FABP4 gene and its association with growth traits of 45 Sistani (n=30) and Dashtiari (n=15) cattle were investigated. DNA extraction from the whole blood was performed and its quality was determined by electrophoresis of one percent agarose gel. Animal genotypes were ...
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The polymorphism in exon three of FABP4 gene and its association with growth traits of 45 Sistani (n=30) and Dashtiari (n=15) cattle were investigated. DNA extraction from the whole blood was performed and its quality was determined by electrophoresis of one percent agarose gel. Animal genotypes were determined based on polymerase chain reaction (PCR) products and their band size electrophoresed on agarose 2.8 percent resulted from enzyme digestion by NlaIII. The pattern of bands showed three genotypes including AA, AB and BB in two Sistani and Dashtiari breeds with frequency of 67, 30 and three percent and 73, 27 and zero percent, respectively. The frequency of A and B alleles in exon three of FABP4 in Sistani and Dashtiari breeds were 82 and 18 percent and 86.5 and 13.5 percent, respectively. Heterozygosity indices including Shannon index (I), Nei’s index, observed and expected heterozygosity in Sistani and Dashtiari population were 48, 30, 30 and 30 percent and 39, 11, 27 and 24 percent, respectively. The association between genotypes and growth-related traits were significant for body weights in six, nine and 12 months of age. Therefore, this locus can be considered as a candidate gene in breeding programs for describing the variation of growth traits after weaning age in calves.